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Objective:To investigate the significance and mechanism of ten-eleven translocation (Tet1) against Mycobacterium marinum ( Mm) infection in mice. Methods:SPF wild-type C57BL/6 and Tet1-knockout (Tet1KO) mice were injected intravenously with Mm. All mice were monitored and the abscesses formed in tail were observed and quantified. Pathological changes in mouse tail tissues were observed using hematoxylin and eosin (HE) staining and transmission electron microscopy and the differences between the two groups were analyzed. Immunohistochemistry staining was used to detect the expression and distribution of TNF-α and TGF-β in mouse tail tissues. Moreover, mouse tail tissues were cultured on 7H10 plates for bacterial counting. The expression of NF-κBp65 and TGF-β was detected by Western blot. Results:Obvious lesions including abscesses and ulcers were formed in the Mm-infected C57BL/6, but only scattered small abscesses were observed in Mm-infected Tet1KO mice. During Mm infection, the bacterial load was gradually increased in C57BL/6 mice, but decreased in Tet1KO mice. Histopathological examination showed that obvious inflammatory cell infiltration and typical granulomatous lesions were found in Mm-infected C57BL/6 mice, while no significant inflammatory cell infiltration was detected in Mm-infected Tet1KO mice. Immunohistochemistry staining demonstrated that the expression of TNF-α and TGF-β was lower in Mm-infected Tet1KO mice than in Mm-infected C57BL/6 mice. Moreover, the expression of phosphorylated NF-κBp65 and TGF-β was significantly reduced in Mm-infected Tet1KO mice as compared with that in Mm-infected C57BL/6 mice. Conclusions:Deletion of Tet1 could alleviate the inflammatory damage mediated by Mm and enhance the host immune response to bacteria.
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OBJECTIVE:To provide reference for rational use of antituberculosis drugs and reduce the occurrence of ADR in multiple-drug resistance tuberculosis (MDR-TB) patients.METHODS:TB inpatients were selected from our hospital during Feb.2012-May 2015,and then divided into suspected MDR-TB group (202 cases) and diagnosed MDR-TB group (162 cases).According to the patient's condition,different treatment management modes were chosen,and the incidence of ADR were observed.Treatment and management period of 2 groups were 18 months.RESULTS:In suspected MDR-TB group,the drug resistance rates to first line drugs were greater than or equal to 26.24％,among which drug resistance rates to isoniazid and rifampicin were about 40％.Resistance rates to second line-drugs were greater than or equal to 4.95％,among which resistance rate to protionamide was the highest,being 46.04％.The results of drug sensitivity tests showed that 44.06％ of patients were sensitive to all first-line drugs,14.36％ of patients were resistant to single drug,9.90％ of patients were usually resistant to drugs and 31.68％ of patients were resistant to multiple drugs.21.78％ of patients were sensitive to all first-line drugs and second-line drugs,24.75％ of patients were resistant to single drug,17.82％ of patients were usually resistant to drugs,32.67％ of patients were resistant to multiple drugs and 2.97％ of patients were extensively resistant to drugs.In the management plan of MDR-TB patients therapy,56.17％ were treated in tuberculosis control institutions;referral to special hospital for treatment accounted for 14.81％;22.22％ didn't receive any treatment;other factors lead to a change in treatment or unable to continue to treat accounted for 6.79％.The incidence of ADR in MDR-TB patients was higher than suspected MDR-TB patients,there was statistical significance between 2 groups (P＜0.05).After the following symptomatic treatment,all patients were improved.CONCLUSIONS:For patients with MDR-TB,according to disease condition,appropriate treatment management program can be found so as to improve therapeutic efficacy,reduce the incidence of ADR,control spreading and development of tubercle bacillus.
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Objective To explore the correlation of lung function and IFN-γ and IL-4 levels in peripheral blood in pulmonary tuberculosis patients. Methods The IFN-γ and IL-4 levels in peripheral blood of 75 pulmonary tuberculosis patients(severe 25 cases,moderate 25 casea,and mild 25 cases) and 30 healthy volunteers were measured by ELISA. Meanwhile FEV1 ,FEV1% and MMEF% of lung function in 75 pulmonary tuberculosis patients were measured.Results The levels of IFN-γ[(0. 204 ±0. 018) μg/L] and IL-4[(0. 523 ±0. 035) μg/L] in peripheral blood were significantly different both between tuberculosis group and control group and among tuberculosis patients ( severe,moderate,and mild) (t =7. 685,6. 374 ,all P ＜0. 05) ;FEV1 ,FEV1% ,and MMEF% of severe and moderate tuberculosis patients were significantly lower than those of mild tuberculosis patients and normal reference value; In tuberculosis patients, FEV1% and MMEF% were negatively related with IL-4 level in peripheral blood( r = -0. 46, -0. 43, all P ＜ 0. 05 ), also significantly positively related with the IFN-γ level in peripheral blood ( r = 0. 47,0. 45, all P ＜0. 05). Conclusion Pulmonary tuberculosis morbility may be due to cellular levels of patients. The IFN-γ and IL-4 levels in peripheral blood could affect the lung function of pulmonary tuberculosis patients.